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transit-x2  (Mirus Bio)


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    Structured Review

    Mirus Bio transit-x2
    Transit X2, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 98/100, based on 2305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transit+x2/TransIT-X2/custom%40mir-6000%4042650208
    Average 98 stars, based on 2305 article reviews
    transit-x2 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Dual knockdown of Alox15 and TGF-β1 by lipid nanoparticle-delivered siRNA in bleomycin-induced pulmonary fibrosis
    Article Snippet: .. The siRNAs were mixed with TransIT-X2 ® (MIR 6000, Mirus Bio, Madison, WI, USA) diluted in Opti-MEM (Thermo Fisher) to a final concentration of 25 nM/well at room temperature for 20 min. ..

    Article Title: Engineering polymeric RNA scaffolds as programmable combinatorial innate immune agonists
    Article Snippet: .. Complexation with Mirus TransIT-X2: 3 μl TransIT-X2 reagent was added to 97 μl solution containing polyRNAs or hybrids dissolved in Opti-MEM, giving a final concentration of 5 or 10 ng/μl (calculated based on the polyRNA concentration in the mixture). ..

    Cell Culture:

    Article Title: Nitrite increases mitofusin-1 levels to inhibit vascular smooth muscle cell proliferation and prevent intimal hyperplasia
    Article Snippet: .. Cells were cultured for 48h prior to transfection with 50 nM siRNA to sramble (Dharmacon D-001810-01-20), Mfn1 (Dharmacon L-099253-02-0010) or March5 (Dharmacon L-085828-02-0005) duplexed with TransIT-X2 (Mirus MIR 6000) in serum-free Opti-MEM. .. Sodium nitrite (0 – 25 μM; Sigma-Aldrich 563218) and PDGF-BB (10 ng/mL; Sigma-Aldrich P3201) were prepared in sterile water and sequentially added to RASMC culture media for 24 or 48h based on the experiment.

    Transfection:

    Article Title: Nitrite increases mitofusin-1 levels to inhibit vascular smooth muscle cell proliferation and prevent intimal hyperplasia
    Article Snippet: .. Cells were cultured for 48h prior to transfection with 50 nM siRNA to sramble (Dharmacon D-001810-01-20), Mfn1 (Dharmacon L-099253-02-0010) or March5 (Dharmacon L-085828-02-0005) duplexed with TransIT-X2 (Mirus MIR 6000) in serum-free Opti-MEM. .. Sodium nitrite (0 – 25 μM; Sigma-Aldrich 563218) and PDGF-BB (10 ng/mL; Sigma-Aldrich P3201) were prepared in sterile water and sequentially added to RASMC culture media for 24 or 48h based on the experiment.

    Article Title: Identification of host lncRNAs that impact Venezuelan equine encephalitis virus TC-83 replication.
    Article Snippet: .. At 24 h after seeding, the cells were transfected with 50 nM siRNA targeting Snhg15 (33) or a pool of four non-targeting siRNAs using TransIT-X2 (Mirus, Mir6000) according to the manufacturer’s protocol, with the exception of using half of the transfection reagent recommended by the manu facturer. .. At 24 h after transfection, both groups of cells were incubated with TC-83 inoculum (MOI 5) for 1 h. After an incubation period, the viral inoculum was replaced with ABM Basal Medium (Lonza, CC-3187) supplemented with AGM SingleQuots (Lonza, CC-4124), and cells were maintained at 37°C and 5% CO2.

    Article Title: Deficiency of GCN5 exacerbates pulmonary fibrosis by disrupting the LKB1-AMPK pathway.
    Article Snippet: Site-directed mutagenesis of FLAG-LKB1 was conducted using the Phusion Flash High-Fidelity PCR master mix (F-548; Thermo Fisher Scientific, USA), following the manufacturer’s instructions. .. TransIT-X2 (MIR6000; Mirus Bio, USA) was used to perform transient transfection. .. In vitro translation In vitro synthesis of protein complexes was performed using the TNT Quick Coupled Transcription/Translation System (L1170; Promega, UK), following the manufacturer’s protocol.

    Article Title: FOXM1 inhibitor, RCM-1, enhances venetoclax mediated apoptosis through downregulation of ATP2B4 in rhabdomyosarcoma
    Article Snippet: .. To knockdown FOXM1 , 76-9 and RH-30 were transfected with pLKO- shFOXM1 plasmid DNA (clone ID: TRCN0000015546; MilliporeSigma) against FOXM1 using TransIT-X2 (Mirus Bio, LLC; cat. no. MIR 6004). .. To overexpress ATP2B4a and ATP2B4b, 76-9 cells were transfected with CMV-ATP2B4a (Origene; cat. no. MC223809 ) and CMV-ATP2B4b (Origene; cat. no. MR215322) or an empty overexpression (OE) plasmid as a control.

    Article Title: Identification of host lncRNAs that impact Venezuelan equine encephalitis virus TC-83 replication.
    Article Snippet: .. At 24 h after seeding, the cells were transfected with 50 nM of siRNA targeting Snhg15 or a pool of four nontargeting siRNAs using TransIT-X2 (Mirus, Mir6000) with the only modification being half of the recommended transfection reagent used. ..


    Knockdown:

    Article Title: FOXM1 inhibitor, RCM-1, enhances venetoclax mediated apoptosis through downregulation of ATP2B4 in rhabdomyosarcoma
    Article Snippet: .. To knockdown FOXM1 , 76-9 and RH-30 were transfected with pLKO- shFOXM1 plasmid DNA (clone ID: TRCN0000015546; MilliporeSigma) against FOXM1 using TransIT-X2 (Mirus Bio, LLC; cat. no. MIR 6004). .. To overexpress ATP2B4a and ATP2B4b, 76-9 cells were transfected with CMV-ATP2B4a (Origene; cat. no. MC223809 ) and CMV-ATP2B4b (Origene; cat. no. MR215322) or an empty overexpression (OE) plasmid as a control.

    Plasmid Preparation:

    Article Title: FOXM1 inhibitor, RCM-1, enhances venetoclax mediated apoptosis through downregulation of ATP2B4 in rhabdomyosarcoma
    Article Snippet: .. To knockdown FOXM1 , 76-9 and RH-30 were transfected with pLKO- shFOXM1 plasmid DNA (clone ID: TRCN0000015546; MilliporeSigma) against FOXM1 using TransIT-X2 (Mirus Bio, LLC; cat. no. MIR 6004). .. To overexpress ATP2B4a and ATP2B4b, 76-9 cells were transfected with CMV-ATP2B4a (Origene; cat. no. MC223809 ) and CMV-ATP2B4b (Origene; cat. no. MR215322) or an empty overexpression (OE) plasmid as a control.

    Modification:

    Article Title: Identification of host lncRNAs that impact Venezuelan equine encephalitis virus TC-83 replication.
    Article Snippet: .. At 24 h after seeding, the cells were transfected with 50 nM of siRNA targeting Snhg15 or a pool of four nontargeting siRNAs using TransIT-X2 (Mirus, Mir6000) with the only modification being half of the recommended transfection reagent used. ..



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